Nature

Comparison of competitive-nested PCR and real-time PCR in detecting BCR-ABL fusion transcripts in chronic myeloid leukemia patients

All peripheral blood or bone marrow samples in this study were derived from CML patients admitted to the MD Anderson Cancer Center from January 1997 to April 2002. Where indicated, only those patient ...
Scientific Research Publishing

Contribution of Nested PCR for the Diagnosis of the Immunodeficiency Virus and the Detection of Endogenization of the Provirus in Serodiscordant Couples in Kinshasa, Democratic ...

1 Makala General Reference Hospital, Kinshasa, Democratic Republic of the Congo. 2 Department of Molecular Biology, Faculty of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of the ...
Frontiers

Identification of Mycobacterium tuberculosis in Clinical Specimens of Patients Suspected of Having Extrapulmonary Tuberculosis by Application of Nested PCR on Five Different Genes

A total of 100 specimens were obtained from clinically suspected EPTB cases on the basis of imaging, clinical findings, histological and cytological observations, admitted to the university teaching ...
Scientific Research Publishing

Comparative Performance of Microscopy and Nested PCR for the Detection of Cryptosporidium Species in Patients Living with HIV/AIDS in Abidjan (Côte d’Ivoire) ()

1 Department of Parasitology Mycology, Institut Pasteur, Abidjan, Côte d’Ivoire. 2 Laboratory of Genetics and Species Improvement, Félix Houphouët-Boigny University, Abidjan, Côte d’Ivoire. 3 ...
International Livestock Research Institute

A nested PCR assay exhibits enhanced sensitivity for detection of Theileria parva infections in bovine blood samples from carrier animals

Theileria parva causes East Coast fever, an economically important disease of cattle in sub-Saharan Africa. We describe a nested polymerase chain reaction (nPCR) assay for the detection of T. parva ...
SciELO

Development of an efficient multiplex semi-nested PCR for convenient use in urine samples for diagnosis of toxoplasmosis

BACKGROUND Toxoplasmosis is a protozoan parasitic infection. The cerebrospinal, ocular and congenital forms of the disease are complicated and life threatening. Diagnosis of toxoplasmosis is difficult ...
C&EN

The End of the Gray Zone: One-Tube Nested Recombinase Polymerase Amplification with Ultrahigh Signal-to-Noise Ratio for Precisely Detecting and Surveilling Viruses

Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, Sichuan 400044, PR China State Key Laboratory of Cattle ...
SciELO

An optimized one-tube, semi-nested PCR assay for Paracoccidioides brasiliensis detection

Introduction Herein, we report a one-tube, semi-nested-polymerase chain reaction (OTsn-PCR) assay for the detection of Paracoccidioides brasiliensis. Methods We developed the OTsn-PCR assay for the ...