Among these methods, real-time PCR (rt-PCR) allow a real-time monitoring of DNA amplification through fluorescent signals proportional to the initial DNA template quantity. This method could serve as ...
Figure 1: Description and proof of principle for balanced PCR. As an alternative to the separation PCR step, LN1- and LN2-ligated plasmid DNA were mixed in different proportions and used in a ...
Acute promyelocytic leukaemia (APL) is characterised by the t(15;17), which produces the PML-RARα fusion gene. 1,2,3 Transcripts for PML-RARα are detected in all patients with t(15;17). The ...
Purpose: To evaluate the sensitivity and accuracy of the quadruple real-time PCR method for the detection of enterococci carrying vancomycin-resistant genes vanA, vanB, and vanM in rectal swabs.
PCR or Polymerase Chain Reaction is a revolutionary method that is used to amplify specific segments of DNA or RNA. This method was developed by Kary Mullis in the 1980s. Kary Mullis received the ...
To diagnose some conditions, such as autoimmune disease, doctors often look for certain antibodies in patients’ blood. Unfortunately, these proteins can be few and far between. Now, researchers have ...
A team of UK-based scientists has recently developed an allele-specific probe polymerase chain reaction method that can accurately detect different variants/lineages of severe acute respiratory ...
Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to ...
Today, PCR is ubiquitous in molecular biology laboratories. As one of the first experiments that young scientists master, it is easy to take for granted. But developing this research lab staple ...
PCR genetic analysis has been in the spotlight since COVID-19, but light is now further facilitating PCR-free methods. Osaka Metropolitan University scientists have developed a light-induced DNA ...
Introduction Occurrence of diverse human enteric bacterial, viral and protozoal pathogens in improved drinking water because of pathogenic microbial contamination is of increasing public health ...
One of the best ways to figure out how far a virus like COVID-19 has spread in a community is to test for it. This step-by-step guide explains how coronavirus swab-the-nose-and-throat tests work, and ...
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